FIGURE 9.

The HDX binding patterns of various thrombin ligands. Thrombin segments that were protected from solvent exchange following 10 min of deuteration are colored in red. The observed HDX effects were statistically different from the values obtained with free thrombin at a level of p ≤ 0.001. The individual ligands bound to thrombin including GpIbα(269–286) (Protein Data Bank code 1P8V), Hirudin(54–65) (Protein Data Bank code 1HAH), and PAR3(44–56) (Protein Data Bank code 2PUX) are depicted as black sticks. The figure was prepared with PyMOL.