FIGURE 3.

The B domain of the regulatory subunit of PKA-RIIα interacts with Smad4. Shown is a schematic of the regulatory subunit of PKA and its truncation mutants (A). Mv1Lu cells were transiently transfected with PKA-RIIα full-length or deletion mutant-expression plasmids in the presence or absence of 100 pm TGF-β for 30 min. Co-immunoprecipitation (IP) assays were performed using anti-Smad4 and V5 antibodies (B). The data shown are representative of three independent experiments with similar results. IB, immunoblot.