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. 2013 Feb 5;288(12):8772–8784. doi: 10.1074/jbc.M112.421057

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Leishmanial-conditioned medium neutralizes trialysin-mediated killing of T. cruzi and Leishmania but shows selective bio-energetic activation for T. cruzi. A, the conditioned medium from T. cruzi (TC) epimastigotes, L. amazonensis (Lma) and L. major (Lmj) promastigotes (as indicated) protect T. cruzi parasites from the parasiticidal effects of P6-trialysin and induce reductase activity (also shown in Fig. 4C), yet they have no effect on the neutralization of the Pro → Ala mutant of trialysin (Mut-T). Black and gray bars indicate WT and mutant trialysin peptides, respectively, compared with untreated control cells (white). B, the effect of P6-T on L. amazonensis with different conditioned-M199 shows that conditioned medium protects parasites from P6-T (black)- but not Mut-T (gray)-induced killing but does not induce reductase activation. The same trends were found using recombinant tCyp19 (not shown). Non-conditioned M199 (NCM) was used as a control (white).