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. Author manuscript; available in PMC: 2013 Mar 22.
Published in final edited form as: Nat Cell Biol. 2001 Feb;3(2):107–113. doi: 10.1038/35055000

Figure 5.

Figure 5

Proteins associated with chromatin in different extracts.

Sperm nuclei were incubated for 20 mins in either metaphase extract, interphase extract, in metaphase extract supplemented with 0.5 or 3 mM 6-DMAP and 0.3mM CaCl2 (“metaphase+6-DMAP”), or in interphase extract supplemented with 7 or 35 nM geminin. Chromatin was isolated and immunoblotted for Orc1, Cdc6, Cdt1, Mcm7 and geminin.