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Effect of TPGS concentration (A), cationic lipid (B) and HSA coating (C, D) on phrGFP downregulation in phrGFP-transfected MCF-7 cells. Cells were treated with different LNPs-siRNA for 4 h at 37°C and then cultured for 48 h at 37°C. Fluorescence intensity of the cell lysates were determined by fluorescence spectrometry. The fluorescence intensity was normalized with the protein content.
