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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biotechniques. 2013 Jan;54(1):25–34. doi: 10.2144/000113981

Figure 1. Schematic diagram of homopolymer tail-mediated ligation PCR (HTML-PCR).

Figure 1

Details of each numbered step are provided in the text. The sample double-stranded DNA sequence is indicated by the thick double lines and is not to scale. For simplicity only a single double-stranded DNA molecule is depicted and only one strand is shown for the PCR step. X and Y represent user-defined oligonucleotide sequences and X′ and Y′ are their respective complementary sequences. C, 2’-deoxycytosine monophosphate; G, 2’-deoxyguanosine monophosphate; ddC, 2′,3′-dideoxycytosine monophosphate.