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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biotechniques. 2013 Jan;54(1):25–34. doi: 10.2144/000113981

Figure 3. Thermodynamic advantage of 2-amino deoxyadenosine-tailed substrates.

Figure 3

HTML-PCR products generated using 100 ng of template and 15 cycles of PCR as visualized by 2% agarose gel electrophoresis. Lanes 1–7 were performed using 2-amino deoxyadenosine-tailed substrate, lane 8 contains 2-Log DNA ladder (New England Biolabs, Ipswich, MA) and lanes 9–15 were performed using dA-tailed substrate. In lanes 1–7 and in lanes 8–15, the PCR annealing temperature was progressively increased as follows: 48°C, 52.1°C, 54.7°C, 58.3°C, 62.4°C, 65.7°C, or 68.4°C, respectively.