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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Int J Biol Macromol. 2012 Nov 10;53:42–53. doi: 10.1016/j.ijbiomac.2012.10.030

Fig. 2.

Fig. 2

Normalized UV–Vis absorption spectra of BSA samples at different incubation times for (A) pH 2.0 buffer and (B) pH 9.0 buffer. The time of observation is indicated by the number with arrows pointing to the corresponding spectrum. In (A) the initial (Day 0) absorption spectrum is indicated by the arrow, followed by the inclusion of the remaining spectra spanning the days of the experiment. No significant change can be seen. In (B) only three spectra have been presented, the initial (the number zero corresponds to the initial reading), 4th week and final week (eighth). Normalization was applied to each set of conditions, not the overall collection, so the actual measured optical density of the two conditions did differ, indicating different concentrations.