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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Commun. 2013;4:1332. doi: 10.1038/ncomms2320

Fig. 4. The pharmacological effects of ebselen are mediated by inositol depletion.

Fig. 4

(a) Schematic outlining the experimental protocol used to investigate inositol reversal of behaviours induced by ebselen. Drugs were injected as follows: 1 μL of 0.5 M inositol, 5 mg/kg ebselen and 2 mg/kg amphetamine. (b) Effect of inositol on the ability of ebselen to attenuate rearing, analysed by pre-planned paired t-tests between ebselen and control, n=4-6. (c) Effect of inositol on the ability of ebselen to attenuate amphetamine-induced hyperactivity (mobility) analyzed by pre-planned paired t-tests between ebselen and control, n=5-6. (d) Proton NMR spectra of authentic inositol and brain extracts from mice injected intraperitoneally with either ebselen (10 mg/kg) or hydroxypropyl ß-cyclodextrin(4% w/v, Control). (e), Effect of ebselen (10 mg/kg) on inositol levels in mouse brain. Inositol was quantified by integration of the C1 and C3 peaks (indicated by the bar in d), and analyzed by a pre-planned one-way t-test between ebselen and control, n=4. All error bars represent standard error of the means.