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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Nature. 2012 Sep 26;490(7420):355–360. doi: 10.1038/nature11438

Figure 1. Aged satellite cells cycle more frequently during homeostasis.

Figure 1

a, Doxycycline (Dox) loading and chase strategy of TetO-H2B-GFP mice and FACS plots for No chase and 84-week chase. SC pool has LRC and nonLRC subsets. b, Strategy and FACS plots for aged (Ag) or adult (Ad) no chase and 12-week chase SCs. Vehicle treated SCs (Neg) (n=10,000 cells,6 animals/group). c, BrdU feeding and representative images of SCs stained with anti-Pax7, BrdU and Dapi. d, Representative sections from aged muscle stained with anti-Pax7, Ki67 and laminin. e, Percentage of BrdU+ SCs (n=300 cells,4-6 animals/group). f, Percentage of Ki67+/Pax7+ SCs/section (n=4-6 animals/group). g, h, RTqPCR; myod (g) p27 and spry1 (h) in SCs. (n=10,000 cells/condition performed in triplicate, 5 mice/group). Scale-bar; 20μm, *P<0.05, s.e.m.