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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Nature. 2012 Sep 26;490(7420):355–360. doi: 10.1038/nature11438

Figure 2. Preservation of quiescence protects satellite cell function.

Figure 2

a, Scheme of transplantation assay of 2,000 aged and adult Dox-fed TetO-H2B-GFP SCs into pre-injured Dox-fed wildtype hosts. Representative images of host whole-mount (b) and muscle sections of transplant recipients (c) lower panels: Pax7+/H2B-GFP+ cell, (Scale-bar 10μm). d, e, Number of H2B-GFP+/myonuclei (d) and Sublaminar H2B-GFP+/SCs (e) summed over six consecutive 10μm sections, averaged across three regions of muscle (n= 4 mice/group). f, Representative image of Pax7+, MyoG+, MyoD+, and activated-caspase-3+ (aCasp) SCs after 4-day culture (4d). g-i, Percentage of Pax7+ (g) MyoG+ (h) and aCasp+ SCs (i) after 4d (n=300-600 cells, in triplicate). j, Myogenic and quiescent marker expression (n=3 mice/condition). k-n, Number cells/clone (k) Percent Pax7+ (l), MyoG+ (m) and aCasp+ (n) after 4d (n=300-5=600 cells, in triplicate). o, Transplant strategy of 2,000 LRC and nonLRCs injected into pre-injured Dox-fed wildtype hosts. q, p, Number of H2B-GFP+/myonuclei (p) Sublaminar-H2B-GFP+/SCs (q) summed over six consecutive 10μm section, averaged across three regions of muscle (n=4 mice/group). Scale-bar; (b, f) 25μm c) 200μm. *P<0.05, s.e.m.