Skip to main content
. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Nature. 2012 Sep 26;490(7420):355–360. doi: 10.1038/nature11438

Figure 3. FGF2 is an aged niche factor that induces satellite cells into cycle.

Figure 3

a, Single muscle fibre expression of fgf2 by RT-qPCR (3 reactions performed in triplicate, n=5 mice/group). b, Fgf2 in-situ hybridizations on adult and aged single muscle fibers. Sense probe was used as a background negative control (lower panel). c, Representative longitudinal sections stained with anti-Pax7 (green, white arrow) laminin (white) and FGF2 (red) Dapi (blue) shows Pax7+ SC (white arrow) close to FGF2 (asterisk). d, Percentage of SCs near FGF2+ regions (<20μm) (n=3 animals/group). e, Strategy to assess mitogenic activity of purified muscle fibre (PM) extracts. f, g, Percentage of cycling (Ki67+) adult and aged SCs treated with adult (Ad) or aged (Ag) PM extract and FGF2-blocking antibody (FGF2bl) (f) or SU5402 (g) (n=1000 cells/condition, n=5 animals/group). h, Strategy to inhibit FGF-activity in-vivo via implanted SU5402 coated beads. i, j, Percent BrdU+/SCs (i) and aCasp+/SCs 4d (j) after 4d from Ad and Ag mice with SU5402 treatment (n=300-600 cells/condition, 4-6 mice/group). Scale=20μm. *P<0.05, ± s.e.m.