Skip to main content
. Author manuscript; available in PMC: 2013 Mar 22.
Published in final edited form as: J Immunol. 2012 May 23;189(1):373–380. doi: 10.4049/jimmunol.1200718

Figure 4. Pro-inflammatory mediators enhance GLUT2 and GLUT10 abundance in H441 airway epithelial monolayers.

Figure 4

Proinflammatory mediators enhance GLUT2 and GLUT10 abundance in H441 airway epithelial monolayers. Untreated (control) or PIM treated (for +24, +48 and +72 h) H441 cell monolayers immunostained with GLUT2 (GLUT2 AB) or GLUT10 (GLUT10 AB) antisera and FITC-conjugated secondary antiserum (green). GLUT2 and GLUT10 specificity was verified by comparison against immunoflorescence from cells immunostained as above but in the presence of antigenic blocking peptides (GLUT2 + BP and GLUT10 + BP, respectively). Overlays of GLUT2 AB and GLUT10 AB with corresponding images of cell nuclei stained with DAPI (blue) are also shown (GLUT2 + DAPI and GLUT10 + DAPI, respectively). Images were acquired with a x40 oil immersion lens under identical conditions of illumination intensity and photo-multiplier settings. One set of images from n = 4 experiments is shown