Figure 3.

Identification of Aβ22–35 as a lipid-specific cholesterol-binding motif. (A) Structure–activity study of Aβ–cholesterol interactions. The indicated synthetic Aβ fragment was injected underneath a monolayer of cholesterol prepared at an initial surface pressure π_i of 15 mN·m^–1. After reaching equilibrium, the maximal surface pressure increase was recorded. The data are expressed as the mean ± SD (n = 3). (B) Kinetics studies of Aβ22–35 interaction with monolayers of cholesterol (○), POPC (■), sphingomyelin (□), or GM1 (▲). The data show the real-time changes of surface pressure following injection of 10 μM Aβ22–35 beneath each lipid monolayer prepared at an initial surface pressure of 20 mN·m^–1. Each curve is representative of three separate experiments (SD < 10%).