Figure 7.

Curcumin accelerates α-Syn aggregation. (a) Time dependent CD spectra of α-Syn in the presence and absence of curcumin. α-Syn in the presence of curcumin showing accelerated conversion of random coil to β-sheet conformation during aggregation. (b) Electron micrographs of the aggregates after 3 days of incubation showing fibrillar morphology of α-Syn in the absence (left) and presence (right) of curcumin. Altered morphology of α-Syn aggregates were seen in the presence of curcumin. Scale bars are 500 nm. (c) SDS-PAGE analysis showing different proteinase K digestion pattern by α-Syn fibrils formed in the presence and absence of curcumin. (d) Time dependent AFM analysis of α-Syn aggregation in the presence and absence of curcumin showing accelerated conversion of oligomers to fibrils in the presence of curcumin. Scale bars are 700 nm.