Figure 3.

Segmented linear regression model comparison between age (x-axis) and methylation output ratio (y-axis) within the FREE2 region of 104 FM females with CGG size ranging between 21 and 1500 repeats. The perpendicular-broken line represents the ‘break point’ age estimated using the segmented linear regression model as detailed in Table 3. From birth to the ‘break point’ age there was significant inverse relationship with MOR for CpG 1, 2 and 6/7, but not CpG8/9 and 10–12. At the ages past the ‘break point’ there was no significant relationship with MOR observed for any of the CpG units. The horizontal-broken line represents the maximum MOR value for the control group with CGG<40, with this value indicated next to this line, with an exception of CpG6/7 where one control outlier at the MOR of 0.63 was not considered as the maximum control value. Note: PM/FM mosaic individuals and FM methylation mosaics as determined by methylation-sensitive Southern blot were not included.