Skip to main content
. 2013 Jan 10;22(8):1539–1557. doi: 10.1093/hmg/ddt005

Figure 6.

Figure 6.

Both cell type-specific TBPH LOF and GOF cause trans-synaptic defects. (A) Expression of pre-synaptic marker Syt::GFP (green) and dendritic marker DenMark (red) in upper motor neurons of the adult central brain of 50-day-old flies using the ellipsoid body neuron-specific EB1-Gal4 driver (EB1>Syt::GFP, DenMark). Syt::GFP (green) expression is seen in the pre-synaptic region, whereas DenMark visualizes post-synaptic dendritic compartment. DenMark signal and Syt::GFP are indicated in single panels (B and C, respectively). (D–F) Driving TBPH-RNAi in EB1 neurons leads to downregulation of DenMark (E) and Syt::GFP (F). (G) DenMark signal is significantly reduced in TBPH LOF (EB1>Syt::GFP, DenMark, TBPH-IR). (H) TBPH knockdown significantly downregulates Syt::GFP. (I–K) DenMark (J) and Syt::GFP (K) expression in EB1 neurons of 40-day-old flies. (L and M) TBPH overexpression in EB1 neurons leads to downregulation of DenMark (M) and Syt::GFP (N). (O) DenMark signal is significantly reduced in TBPH GOF (EB1>Syt::GFP, DenMark, TBPH). (P) TBPH overexpression significantly downregulates Syt::GFP. *P < 0.05, **P < 0.01, ***P < 0.001. Mean and SEM are shown. Scale bar: 50 µm.