Table 2.

Summary of two-way ANOVAs followed by Tukey HSD post hoc tests testing the effect of pCO₂ and temperature on Lithophyllum cabiochae metabolism in the dark and at the culture irradiance levels and Chl a content at each season

	ANOVA data
		Source of variation						Tukey HSD test
		pCO2		temperature		pCO2 × temperature
	df	F	P	F	P	F	P	400 T	400 T+3	700 T	700 T+3
Summer
Dark
Rd (O2)	(1,16)	0.030	0.864	0.085	0.775	6.864	0.019	nd
Rd (CO2)	(1,16)	0.153	0.700	0.320	0.579	1.820	0.196	nd
Gd (CaCO3)	(1,16)	0.432	0.520	8.749	0.009	6.913	0.018	a	b	ab	ab
Light
Pn (O2)	(1,16)	33.977	<0.001	6.524	0.021	1.390	0.256	a	a	ab	b
Pn (CO2)	(1,16)	41.200	<0.001	10.160	0.006	2.610	0.126	a	a	ab	b
Pg (O2)	(1,16)	17.983	<0.001	4.243	0.056	3.853	0.067	a	a	ab	b
Pg (CO2)	(1,16)	13.216	0.002	2.747	0.117	3.304	0.088	a	a	ab	b
G (CaCO3)	(1,16)	8.346	0.011	2.743	0.117	24.690	<0.001	a	ab	b	a
Chl a	(1,16)	0.017	0.897	2.895	0.108	0.617	0.444	nd
Autumn
Dark
Rd (O2)	(1,16)	3.552	0.078	7.136	0.017	0.595	0.452	nd
Rd (CO2)	(1,16)	0.035	0.853	12.203	0.003	0.448	0.513	nd
Gd (CaCO3)	(1,16)	0.369	0.552	0.000	0.984	2.824	0.112	nd
Light
Pn (O2)	(1,16)	28.611	<0.001	7.489	0.015	0.139	0.714	a	a	ab	b
Pn (CO2)	(1,16)	10.830	0.005	4.921	0.041	3.157	0.095	a	ab	b	b
Pg (O2)	(1,16)	17.108	<0.001	0.211	0.652	0.039	0.846	nd
Pg (CO2)	(1,16)	7.219	0.017	1.141	0.301	0.792	0.387	nd
G (CaCO3)	(1,16)	0.167	0.689	8.167	0.011	8.167	0.011	ab	ab	a	b
Chl a	(1,16)	1.009	0.330	0.646	0.433	0.091	0.767	nd
Winter
Dark
Rd (O2)	(1,15)	0.257	0.619	18.389	<0.001	0.938	0.348	ab	a	b	a
Rd (CO2)	(1,15)	2.834	0.113	2.262	0.153	1.311	0.270	nd
Gd (CaCO3)	(1,15)	0.017	0.898	3.794	0.070	0.676	0.424	nd
Light
Pn (O2)	(1,15)	62.325	<0.001	7.310	0.016	0.469	0.503	a	a	b	b
Pn (CO2)	(1,15)	48.847	<0.001	0.555	0.468	3.976	0.065	a	a	b	b
Pg (O2)	(1,15)	51.721	<0.001	1.056	0.321	0.000	1.000	a	a	b	b
Pg (CO2)	(1,15)	27.699	<0.001	5.228	0.037	1.082	0.315	ab	a	b	b
G (CaCO3)	(1,15)	0.344	0.566	12.396	0.003	0.344	0.566	nd
Chl a	(1,15)	0.350	0.563	3.963	0.074	1.071	0.317	nd
Spring
Dark
Rd (O2)	(1,15)	0.096	0.761	6.869	0.019	3.565	0.078	nd
Rd (CO2)	(1,15)	0.126	0.728	3.142	0.097	0.005	0.944	nd
Gd (CaCO3)	(1,15)	0.609	0.447	1.007	0.332	0.311	0.585	nd
Light
Pn (O2)	(1,15)	3.371	0.086	0.090	0.768	2.703	0.121	nd
Pn (CO2)	(1,15)	0.143	0.711	0.548	0.470	17.780	0.001	a	b	ab	ab
Pg (O2)	(1,15)	4.235	0.057	0.448	0.514	4.661	0.047	nd
Pg (CO2)	(1,15)	0.017	0.898	3.625	0.076	12.398	0.003	a	b	ab	ab
G (CaCO3)	(1,15)	1.734	0.208	0.444	0.515	7.864	0.013	nd
Chl a	(1,15)	0.024	0.880	4.575	0.049	0.062	0.807	nd

R_d, dark respiration; P_n, net production; P_g, gross production; G_d, net calcification in the dark; G, net calcification in the light. Bold type indicates Bonferroni-adjusted significance (P < 0.0125). Different letters (a and b) indicate significant difference between treatments: 400 T, 400 T + 3, 700 T, and 700 T + 3 (P < 0.0125, Tukey's HSD test); nd, no difference.