Figure 3.

Effects of compounds on [Ca²⁺]_i in T-REx FFA1 cells and Flp-in GPR120 cells. T-REx FFA1 cells and Flp-in GPR120 cells were stimulated with α-LA (A), NCG75 (B) or compound 1 (C). Cells were seeded at a density of 2 × 10⁵ cells per·well on collagen-coated 96-well plates, incubated at 37°C for 21 h and then incubated in HBSS (pH 7.4) that contained Calcium Assay Kit Component A for 1 h at room temperature. Compounds that were used in the FLIPR assay were dissolved in HBSS (1% DMSO) and prepared in another set of 96-well plates. These plates were set on the FLIPR, and mobilization of [Ca²⁺]_i evoked by agonists was monitored.