Figure 4.

The ENaC/Deg-like channel is activated by exposure to hypertonic solutions. (A) Cells were initially equilibrated in 309mOsm and then switched to 489mOsm as indicated by the bar. Osmolarity was increased with addition of sucrose. An ensemble average of single channel activity is shown (n = 9 patches); the mean increase in current was 4.67 ± 1.7 pA (n = 8, P ≤ 0.05), and this increase was not seen in the presence of 1 μm benzamil. (B) Representative section taken from one of the 309mOsm records averaged in panel A. (C) Representative section taken from one of the 489mOsm records averaged in panel A.