Table 1.

Extracellular solutions used during electrophysiology experiments

Extracellular (mM)	Na	K	Ca	Cl	Mg	Cs	SO4	MeSO4	Li	Glucose	HEPES	pH 7.4	Vj (mV)
1. Inside-out patch	155	0	4	158	0	0	0	0	0	0	10	NaOH	−5.9
2. Lithium solution	0	9	2	151	1	0	0	0	140	0	10	KOH	−6
3. Cell-attached patch bath solution	0	115	2	122.2	1.6	0	0	0	0	10	10	KOH
4. Physiological saline	145	5	2	151	1	0	0	0	0	0	10	NaOH	−14.4
5. NaMeSO4	155	0	2	4	0	0	0	150	0	0	10	NaOH	0.5
6. ENaC permeability	158	5	2	159	0	0	0	0	0	0	10	NaOH	a

Vj values quoted are when matched with the appropriate intracellular solutions (Table 2). Calculations were performed with JpCalc (Barry and Lynch, 1991). Osmolarity was adjusted in all cases by addition of sucrose as appropriate. Sucrose was chosen to adjust osmolarity so that the ion concentrations remained unchanged during any given experiment.

^aVj varied between 0.1 and 4 mV when paired with intracellular solutions with different sodium concentrations. NB All ion concentrations are the total number of ions in the solution after adjustment of pH with NaOH or KOH as appropriate.