Table 2.
Intracellular solutions used during electrophysiology experiments
| Intracellular (mM) | Na | K | Li | Ca | Cl | Mg | Cs | SO4 | MeSO4 | Gluconate | EGTA | BAPTA | TEA | HEPES | pH 7.2 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1. Inside-out patch | 188 | 0 | 0 | 0 | 10 | 0 | 0 | 90 | 0 | 0 | 5 | 0 | 10 | 10 | NaOH |
| 2. Lithium solution | 5 | 5 | 140 | 0 | 145 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | NaOH |
| 3. Cell attached patch bath solutiona | |||||||||||||||
| 4. Physiological salineb | 0 | 150 | 0 | 0 | 28 | 1 | 0 | 0 | 0 | 115 | 5 | 0 | 0 | 10 | KOH |
| 5. NaMeSO4 | 160 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 150 | 0 | 0 | 5 | 0 | 10 | NaOH |
| 6. ENaC Permeability | c | c | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 10 | NaOH |
a
No intracellular solutions in this configuration.
b
No intracellular solutions were used for the volume recording experiments since cells were intact.
c
[Na] and [K] were varied between 22 and 150 mM in these experiments to determine their relative permeability (see Figure 3). All ion concentrations are the total number of ions in the solution after adjustment of pH with NaOH or KOH as appropriate.