Figure 1.

M2 inhibition of hepatic fat accumulation and body weight gain. (A) Oil Red O staining. Male C57BL/6 mice were fed on either a normal diet (ND) or high-fat diet (HFD) for 8 weeks. M2 (10 or 30 mg·kg⁻¹·day⁻¹) was orally administered to mice four times per week during the last 4 weeks of the diet feeding. Control animals received vehicle only. The representative pictures show Oil Red O staining of the liver sections (magnification 100×). (B) Hepatic TG contents (n = 5–9 animals per group). (C) Body weight gains. At the end of HFD feeding, mice were fasted overnight to measure fasting plasma glucose levels. Fasting caused decreases in body weight at the eighth week. (D) Plasma TG, total cholesterol and fasting glucose contents. For B, C and D, **P < 0.01, significantly different from ND alone; ^#P < 0.05, ^##P < 0.01, significantly different from HFD alone. For C and D, n = 7–12 animals per group.