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. 2013 Mar 12;168(7):1662–1674. doi: 10.1111/bph.12064

Table 1.

Saturation bioluminescence resonance energy transfer between CXCR3 and CXCR4

BRET50 BRETmax
CXCR4-Rluc/CXCR4-EYFP 2.73 ± 0.09 1.06 ± 0.011
CXCR4-Rluc/CXCR3-EYFP 3.58 ± 0.17 0.35 ± 0.005
CXCR3-Rluc/CXCR4-EYFP 4.11 ± 0.14 0.18 ± 0.002
CXCR3-Rluc/CXCR3-EYFP 1.97 ± 0.09 0.33 ± 0.004

HEK293T cells were transfected with a constant amount of CXCR3-Rluc or CXCR4-Rluc DNA (150 ng/106 cells) and increasing amounts of CXCR3-EYFP or CXCR4-EYFP DNA (10–2200 ng/106 cells). BRET50 and BRETmax values ± SE were determined by fitting pooled data from three or more independent experiments to a single binding site isotherm.