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. 2013 Mar 12;168(7):1707–1718. doi: 10.1111/bph.12054

Figure 5.

Figure 5

Effects of andrographolide on nuclear Nrf2 and cellular GSH levels. (A) Cytoplasmic and nuclear extracts of BEAS-2B cells treated with 2% CSE in the presence and absence of 30 μM andrographolide for 4 h and 24 h were separated in 10% SDS-PAGE. Immunoblots were probed for Nrf2. β-actin and TBP were used as internal controls for cytosolic and nuclear proteins respectively. The experiments were repeated four times with similar pattern of results. Protein band intensities were analysed using ImageJ software and were normalized to β-actin controls. Values shown are the mean ± SEM. (B) ARE-binding activity of Nrf2 in nuclear extracts of BEAS-2B cells stimulated with 2% CSE in the presence and absence of 30 μM andrographolide for 4 h and 24 h was determined using a TransAM™ Nrf2 elisa kit. (C) Cellular GSH levels at 24 h after exposure to 2% CSE in the presence and absence of 30 μM andrographolide were measured using a GSH assay kit. Experiments were repeated four times, and values are expressed as means ± SEM. #Significant different from control and *significant difference from DMSO, P < 0.05.