Fig. 3.
Prenatal ethanol increases the density of peptide-expressing neurons in P0 offspring prenatally exposed to 1 g/kg or 3 g/kg ethanol relative to control (n = 6–8/group), as assessed by in situ hybridization histochemistry with digoxigenin-labeled probes. (A) Density of peptide-expressing neurons. Data are mean ± SEM, *p < 0.05, vs. control. (B) Photomicrographs illustrating this effect of 1 g/kg ethanol vs. control group on ENK, GAL and OX (10× magnification). Scale bar in left panel = 200 μm.