Figure 2.

Treatment with sera collected from caloric-restricted (CR) Macaca mulatta significantly increases proliferation capacity of human coronary arterial endothelial cells (CAECs). Cell proliferation capacity was assessed in CAEC stimulated with vascular endothelial growth factor (VEGF; 100ng/mL) using the flow cytometry–based Guava Cell Growth assay (see Methods section). The inverse of the fluorescence intensity of the indicator dye carboxyfluorescein diacetate succinimidyl ester (CFSE) was used as an index of proliferation capacity of the cells. Data are means ± SEM (n = 5 in each group), *p < .05 versus control.