Figure 5.

(A) Treatment with sera collected from caloric restricted (CR) Macaca mulatta significantly inhibits apoptosis in human coronary arterial endothelial cells (CAECs). Apoptotic cell death was assessed by measuring caspase 3/7 activity in cell lysates. *p < .05 versus control serum treated. Data are mean ± SEM. (n = 5 for each group). (B) In cultured CAECs treatment with CR sera did not alter cellular NO production, as compared with cells grown in the presence of sera obtained from control animals. Cellular NO production was assessed using the triazolofluorescein (DAF-2T) fluorescence method. Data are mean ± SEM. (n = 5 for each group).