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. 2012 Aug 17;68(3):235–249. doi: 10.1093/gerona/gls158

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© The Author 2012. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Figure 6. — (A) Reporter gene assay showing the effects of treatment with sera collected from caloric-restricted (CR) Macaca mulatta on Nrf2/ARE reporter activity in cultured primary human coronary arterial endothelial cells (CAECs). Cells were transiently cotransfected with ARE-driven firefly luciferase and CMV-driven renilla luciferase constructs. At the end of the culture, period cells were then lysed and subjected to luciferase activity assay. After normalization, relative luciferase activity was obtained from four to six independent transfections. Data are mean ± SEM (n = 5 for each group). (B–E). Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) data showing the effect of treatment with CR sera on mRNA expression of NFE2L2 (Nrf2), NQO1, HMOX1, and GCLC in cultured primary CAECs. Data are mean ± SEM. (n = 5 in each group). (F) Effect of treatment with CR sera on mitochondrial production in CAECs, assessed using the MitoSOX Red fluorescence method. Data are mean ± SEM. (n = 5 for each group).

Inline graphic — (A) Reporter gene assay showing the effects of treatment with sera collected from caloric-restricted (CR) Macaca mulatta on Nrf2/ARE reporter activity in cultured primary human coronary arterial endothelial cells (CAECs). Cells were transiently cotransfected with ARE-driven firefly luciferase and CMV-driven renilla luciferase constructs. At the end of the culture, period cells were then lysed and subjected to luciferase activity assay. After normalization, relative luciferase activity was obtained from four to six independent transfections. Data are mean ± SEM (n = 5 for each group). (B–E). Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) data showing the effect of treatment with CR sera on mRNA expression of NFE2L2 (Nrf2), NQO1, HMOX1, and GCLC in cultured primary CAECs. Data are mean ± SEM. (n = 5 in each group). (F) Effect of treatment with CR sera on mitochondrial production in CAECs, assessed using the MitoSOX Red fluorescence method. Data are mean ± SEM. (n = 5 for each group).