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Treatment with sera collected from caloric restricted (CR) Macaca mulatta upregulates vascular endothelial growth factor (VEGF) signaling in human coronary arterial endothelial cells (CAECs). The time course for phosphorylation of SAPK/JNK (A), p38 MAPK (B), Akt (C), and HSP27 (D) in CAECs exposed to 100ng/mL VEGF for 5, 15, and 30 minutes was shown. Protein phosphorylation was analyzed using a magnetic multiplex bead array (Millipore; see the Methods section).
