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. 2013 Mar 21;8(3):e59740. doi: 10.1371/journal.pone.0059740

Figure 2. Formation of haem deposits and fluorescent deposits after needle stick lesion.

Figure 2

A–D: Prussian blue-labelled (haem) deposits, 3–30 d after lesion. Deposits formed along the needle track, and were tightly confined to the track. They were relatively diffuse at 3 d, and became dense by 7 d. E–H: Fluorescent deposits along the needletrack 7–30 d after the lesion. Like haem deposits, the fluorescent deposits were distributed along, and confined to the needle track (H). The red label shows synaptophysin labelling in E, and astrocytes (GFAP) in F–H. The GFAP labelling is tightly confined to the needletrack in H. The blue fluorescence is bisbenzimide labelling of nuclear DNA. The needle track in H is through the hippocampus; GrDG indicates the granule layer of the dentate gyrus.