Table 1. Antibodies used for Western Blotting (WB) and immunohistochemistry (IHC).
Antigen/name | Epitope/Clone | Host species | Working dilution IHC/(WB) | Supplier & Catalogue no. | |
Primary Antibodies | Alzheimer precursor protein | N-terminal pre-A4/22C11 | Mouse | 1∶200 (2500) | Millipore MAB348 |
β-Amyloid | Aβ1–16/6E10 | Mouse | 1∶500 (1000) | Covance SIG39320 | |
β-Amyloid | Aβ17–24/4G8 | Mouse | 1∶500 (1000) | Covance SIG39220 | |
Aβ oligomers | Prefibrillar oligomers/A11 | Rabbit | 1∶1350 (500) | Millipore AB9234 | |
CD11b | OX-42 | Mouse | 1∶50 | Millipore CBL1512 | |
GFAP | − | Rabbit | 1∶1000 | Dako Z0334 | |
Neuronal Nuclei (NeuN) | A60 | Mouse | 1∶100 | Millipore MAB377 | |
Synaptophysin | p38 | Rabbit | 1∶200 (10000) | Dako A0010 | |
Phosphorylated Tau | AT8 | Mouse | 1∶500 | Innogenetics 90206 | |
GAPDH | − | Mouse/Rabbit | (1∶3000) | Sigma G8795/9545 | |
Secondary Antibodies | Alexa Fluor 488-conjugated | Mouse IgG | Goat | 1∶1000 | Molecular Probes A11029 |
Alexa Fluor 594-conjugated | Rabbit IgG | Goat | 1∶1000 | Molecular Probes A11037 | |
Biotinylated | Mouse IgG | Goat | 1∶200 | Vector BA9200 | |
HRP-conjugated | Mouse/Rabbit IgG | Goat | (1∶3000) | Millipore AP308/307 |
Working concentrations for Western Blotting are indicated in brackets.