Table 3.
Quantitation of the intensity of complex IV active elements and mitochondrial mass within axons
|
|
|||||
|---|---|---|---|---|---|
| Control (SMI31) | NAWM (SMI31) |
Lesion (APP) |
Lesion (SMI32) |
Lesion (SMI31) |
|
|
|
|||||
| Complex IV (intensity) brain |
17.93 ± 5.89 (76) |
16.14 ± 6.54 (76) |
9.85 ± 6.94# (37) |
10.26 ± 5.43# (58) |
20.10 ± 7.38†,‡ (98) |
| Complex IV (intensity) spinal cord |
16.99 ± 15.49 (105) |
16.87 ± 12.44 (87) |
9.01 ± 11.16# (38) |
15.93 ± 13.01 (49) |
23.04 ± 11.07* (89) |
| Porin (percentage area) brain |
6.74 ± 2.82 (64) |
7.35 ± 4.30 (73) |
7.93 ± 4.50 (32) |
4.19 ± 3.95† (43) |
11.15 ± 5.23* (92) |
| Porin (percentage area) spinal cord |
5.93 ± 4.38 (138) |
3.46 ± 3.92 (135) |
8.26 ± 7.40 (45) |
1.64 ± 1.39# (55) |
13.97 ± 7.23* (132) |
The intensity of complex IV active elements represents the difference in densitometric value between background and complex IV active elements in inverted grey scale 100× brightfield images of cytochrome c oxidase or COX histochemistry. The percentage area of porin reactive elements within axons was calculated based on the total area of axonal porin reactive elements in triple labelled (porin, syntaphilin and axonal marker) and area of axons in confocal images.
†
P = 0.001 (versus NAWM) and
‡
P = 0.002 (versus CON).
#
P < 0.001 (versus SMI31 in CON, NAWM and lesion).
*
P < 0.001 (versus SMI31 in NAWM and CON as well as SMI32 in lesion and APP in lesion).