FIGURE 5.

(A) The extracted ion chromatogram for m/z 752.30, which corresponds to the BSA peptide NYQEAK, obtained with the fast-gradient conditions. The extracted ion chromatogram contains a narrow chromatographic peak for this peptide. These data also show that no MS/MS spectra were collected on this peptide while it was eluting. (B) A mass spectrum collected during the elution of peptide NYQEAK, showing that other ions, several of which had higher abundance, were present, along with the ion of interest at m/z 752.30.