Table 4.

Mascot Search Results from LC-MS/MS Analysis of Tryptic-Digested BSA Using Different Dynamic Exclusion Times

Application of preferred settings	Condition change	Mascot score	Sequence coverage	Matched spectra	Peptides identified	Spectra/peptide identification
S.P.P. 50 mm/20 min	DX = 15 s	2749	83%	248	83	3.0
S.P.P. 50 mm/20 min	DX = 30 s	2845	83%	221	89	2.5
S.P.P. 50 mm/20 min	DX = 60 s	1699	82%	152	74	2.1
S.P.P. 50 mm/20 min	DX = 120 s	1385	72%	114	64	1.8

Data were obtained using the optimum DDA settings determined in Table 3. Specifically, minimum MS signal 1000, repeat count one, and repeat duration 10 s were applied.