Table 5.
Proteomic Results from T. brucei Analysis under Various Chromatographic Conditions with the Application of Optimized DDA Settings
| Column | Flow rate | Exp. time | Protein identifications | Matched spectra | Peptide identifications | Spectra/peptide | Peptide/min |
|---|---|---|---|---|---|---|---|
| Porous 50 mm | 4 μL/min | 90 min | 22 | 370 | 121 | 3.1 | 2.1 |
| Porous 50 mm | 9 μL/min | 21 min | 15 | 376 | 86 | 4.4 | 9.4 |
| S.P.P. 50 mm | 4 μL/min | 90 min | 43 | 439 | 139 | 3.2 | 2.4 |
| S.P.P. 50 mm | 9 μL/min | 21 min | 45 | 477 | 185 | 2.6 | 11.9 |
Results from the analysis of soluble proteins from T. brucei using all four experimental conditions examined. Each condition displays data from duplicate analysis with protein and peptide identifications validated using a 5% false discovery rate. Peptide/min reflects the total number of peptides identified for each condition divided by total experiment time for duplicate sample analysis.