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Overexpression of epidermal growth factor 2 leads to strong, complete staining of the tumor cell membrane according to the HercepTest scoring system (DakoCytomation, Glostrup, Denmark). Intratumoral necrosis is also observed (asterisk). EnVision horseradish peroxidase immunohistochemistry system (REAL EnVision Kit; DakoCytomation) with hematoxylin counterstain. Bar = 36 μm.
