Fig. 1.

A transgenic UPase1 knock-in mouse strain (UPase1-TG) with depleted endogenous uridine level. (A) Elevated UPase1 protein expression in the liver tissues of UPase1-TG mice compared with wild-type mice was observed with Western blot analysis. (B–D) Compared with wild-type mice, UPase1-TG mice exhibited (B) higher liver UPase enzymatic activity, (C) lower liver uridine concentration, and (D) higher liver β-alanine concentration. Error bars are standard deviations of measurements of three mice per animal group. *P < 0.05, **P < 0.01, ***P < 0.001 versus WT control. P values were calculated using Excel's paired Student t-test function.