TABLE 2.
Uridine induces changes to the protein acetylation profile
| Spot Number | Protein Name | WT | WT + Uridine | UPase1-TG | UPase1-TG + Uridine | Biological Process |
| 1 | SEC14-like protein 2 | √ | √ | — | √ | Cellular transport |
| 2 | Fumarylacetoacetase | √ | √ | — | √ | Amino acid metabolism |
| 3 | Isocitrate dehydrogenase [NADP] | √ | √ | — | √ | TCA cycle |
| 4 | Argininosuccinate synthase | √ | √√ | √ | √√ | Amino acid metabolism |
| 5 | Argininosuccinate synthase | √ | √√ | √ | √√ | Amino acid metabolism |
| 6 | Glycine N-methyltransferase | √ | √ | √ | √ | Amino acid metabolism |
| 7 | Carbonic anhydrase 3 | √ | √ | √ | √ | Acid-base balance |
| 8 | Carbonic anhydrase 3 | √ | √ | √ | √ | Acid-base balance |
| 9 | Superoxide dismutase [Mn] | √ | √ | √ | √ | Antioxidation |
| 10 | Peroxisomal bifunctional enzyme | √ | √√ | √ | √ | Lipid metabolism |
| 11 | Acyl-CoA thioesterase 1 | √ | √ | √ | √ | Lipid metabolism |
| 12 | Arginase-1 | √ | √ | √ | √ | Urea cycle |
| 13 | 3-ketoacyl-CoA thiolase B | √ | √√ | √ | √√ | Lipid metabolism |
| 14 | Elongation factor 1-α 1 | √ | √√ | √ | √√ | Protein translation |
| 15 | Cytochrome c1, heme protein | √ | √ | √ | √ | Electron transport |
| 16 | L-xylulose reductase | √ | √ | √ | √ | Glucose metabolism |
| 17 | D-β-hydroxybutyrate dehydrogenase | √ | √√ | √ | √√ | Ketone body metabolism |
| 18 | Protein NipSnap homolog 1 | √ | √√ | √ | √√ | Unknown |
| 19 | Peroxisomal acyl-CoA oxidase 1 | √ | √ | √ | √√ | Lipid metabolism |
| 20 | Fructose-bisphosphate aldolase B | √ | √√ | √ | √√ | Glycolysis |
| 21 | Glyceraldehyde-3-phosphate dehydrogenase | √ | √√ | √ | √√ | Glycolysis |
| 22 | Uricase | √ | √√ | √ | √√ | Purine metabolism |
| 23 | Glutamate dehydrogenase 1 | — | √ | — | — | Amino Acid metabolism |
| 24 | Malate dehydrogenase | — | √ | — | — | TCA cycle |
| 25 | Dihydropyrimidinase | — | √ | — | — | Pyrimidine metabolism |
| 26 | Catalase | — | √ | — | √ | Antioxidation |
| 27 | Delta-1-pyrroline-5-carboxylate dehydrogenase | — | √ | — | √ | Urea/TCA cycle |
| 28 | Methylmalonate-semialdehyde dehydrogenase | — | √ | — | √ | Pyrimidine metabolism |
| 29 | Catalase | — | √ | — | √ | Antioxidation |
| 30 | Hydroxymethylglutaryl-CoA synthase | — | √ | — | √ | Steroid metabolism |
| 31 | Alcohol dehydrogenase 1 | — | √ | — | √ | Oxidation-reduction |
| 32 | Betaine homocysteine S-methyltransferase 1 | — | √ | — | √ | Amino acid metabolism |
| 33 | Superoxide dismutase [Mn] | — | √ | — | √ | Antioxidation |
| 34 | Glutathione S-transferase | — | √ | √ | √ | Antioxidation |
| 35 | Regucalcin | — | — | √ | √ | Calcium signaling |
| 36 | Heat shock cognate 71 kDa protein | — | — | √ | √ | Stress response |
| 37 | ATP synthase subunit β | — | — | √ | √ | ATP biosynthesis |
| 38 | Actin, cytoplasmic 2 | — | — | √ | √ | Cytoskeleton |
| 39 | Alcohol dehydrogenase 1 | — | — | √ | √ | Oxidation-reduction |
| 40 | Carbonic anhydrase 3 | — | — | √ | √ | Acid-base balance |
| 41 | Retinal dehydrogenase 1 | — | — | — | √ | Retinol metabolism |
| 42 | Glutathione S-transferase | — | — | — | √ | Antioxidation |
| 43 | Retinal dehydrogenase 1 | — | — | — | √ | Retinol metabolism |
| 44 | Cytochrome b-c1 complex subunit 2 | — | — | — | √ | Electron transport |
| 45 | L-lactate dehydrogenase A | — | — | — | √ | Oxidation-reduction |
| 46 | Alcohol dehydrogenase 1 | — | — | — | √ | Oxidation-reduction |
| 47 | Glyceraldehyde-3-phosphate dehydrogenase | — | — | — | √ | Glycolysis |
| 48 | Uricase | — | — | — | √ | Purine metabolism |
| 49 | Estradiol 17 β-dehydrogenase 5 | — | — | — | √ | Steroid metabolism |
| 50 | Guanine nucleotide-binding protein subunit β-2-like 1 | — | — | — | √ | Cellular signaling |
| 51 | Glycine N-acyltransferase-like protein | — | — | — | √ | Amino acid metabolism |
| 52 | Carbonic anhydrase 3 | — | — | — | √ | Acid-base balance |
| 53 | Electron transfer flavoprotein subunit β | — | — | — | √ | Electron transport |
| 54 | Betaine homocysteine S-methyltransferase 1 | — | — | — | √ | Amino acid metabolism |
| 55 | Argininosuccinate synthase | — | — | — | √ | Amino acid metabolism |
—, absence of acetylated protein; √, presence of acetylated protein; √√, increase in expression of acetylated protein due to uridine supplementation; bold font, proteins with multiple spots on 2-D gel; highlighted box, proteins acetylated due to uridine supplementation in both wild-type and UPase1-TG mice. WT, wild-type.