Fig. 3.

Moderate cholesterol depletion suppresses BODIPY-SM and BODIPY-PC, but not BODIPY-GlcCer, domains and induces DiIC18 patches. Normal RBCs were treated in suspension with the indicated mβCD concentrations (for level of depletion and lack of toxicity, see supplementary Fig. IIIA), then attached onto PLK-coverslips for 4 min, spread for an additional 4 min, labeled with BODIPY-GlcCer (a–d), BODIPY-SM (e–h), BODIPY-PC (i–l), or DiIC18 (m–p), rinsed, and imaged as in Fig. 1A. Notice the disappearance of BODIPY-SM and BODIPY-PC domains under moderate (∼25%) cholesterol depletion (g, h, k, l) contrasting with better preservation of BODIPY-GlcCer domains (a–d), and conversely, induction of DiIC18 patches (m–p). Scale bar, 5 μm. For general views, see supplementary Fig. IIIB. *, BODIPY.