Fig. 6.

Body weights and epididymal fat mass. (A) Nine-week-old male MLpLKO (filled squares, n = 11) and control fLpL (open squares, n = 8) mice were fed a HFD for 10 weeks and weighed weekly. (B) At 14 weeks of age, ApoEKO (n = 15) and MLpLKO/ApoEKO (n = 17) mice maintained on a chow diet were switched to a WTD for 12 weeks. Body weights and epididymal fat mass were determined at the end of the WTD feeding. (C) Histology of epididymal adipose tissue. The average size of lipid droplet was determined in ApoEKO (n = 5) and MLpLKO/ApoEKO (n = 5) mice fed a WTD for 12 weeks. (D) Gene expression levels in adipose tissue of mice fed a WTD for 12 weeks beginning at age 14 weeks. Identically aged mice maintained on chow were used as controls. Total RNA was extracted from epididymal fat in ApoEKO (open bars, chow; n = 5, WTD; n = 5–8) and MLpLKO/ApoEKO (filled bars, chow; n = 5, WTD; n = 5–8) mice, and gene expression levels were determined by real-time PCR. *P < 0.05, **P < 0.01, ***P < 0.001.