Fig. 3.

Two independent target sites for LDs are present in NS4B. (A) Schematic representation of the intact and mutant NS4B. NS4B proteins were N-terminally tagged with mCherry protein. Numbers indicate the amino acid position in NS4B, and mt denotes the alanine substitution construct. (B) Localization of the N-terminal, central, and C-terminal domains of NS4B. Oc cells were transfected with Cherry-4B1–73, Cherry-4B74–191, or Cherry-4B192–261 for 24 h, and then fixed and stained with Bodipy 493/503. (C, D) Localization of the subdivided N- and C-terminal domains of NS4B. (B–D) Scale bars, 10 μm.