TABLE 1.
Category (% Cells) |
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Construct | I | II | III | IV |
Cherry-4B1–261a | 3.7 ± 1.5 | 15 ± 7 | 11 ± 4 | 71 ± 9 |
Cherry-4B1–73a | 71 ± 12 | 29 ± 12 | 0 | 0 |
Cherry-4B74–191 | 0 | 0 | 0 | 100 |
Cherry-4B192–261a | 14 ± 7 | 34 ± 5 | 22 ± 9 | 30 ± 8 |
Cherry-4B1–49 | 0 | 0 | 0 | 100 |
Cherry-4B26–73 | 15 | 45 | 21 | 19 |
Cherry-4B1–25 | 0 | 0 | 0 | 100 |
Cherry-4B26–49 | 0 | 0 | 0 | 100 |
Cherry-4B50–73 | 0 | 2 | 21 | 77 |
Cherry-4B192–239 | 0 | 0 | 2 | 98 |
Cherry-4B218–261 | 0 | 0 | 10 | 90 |
Cherry-4B192–217 | 0 | 0 | 0 | 100 |
Cherry-4B218–239 | 0 | 0 | 0 | 100 |
Cherry-4B240–261 | 0 | 0 | 8 | 92 |
pcDNA-4B1–261-FLAG | 0 | 3 | 5 | 93 |
The degree of LD targeting for each cell is expressed as category I, II, III, or IV. Each construct was transfected into Oc cells, and the percentages of cells with each category are shown.
Results are mean ± SD from three independent experiments.