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. 2013 Apr;54(4):923–935. doi: 10.1194/jlr.P031260

Fig. 9.

Fig. 9.

Enzyme-dependent formation of oxygenated metabolites of EPA and DHA is independent of stimulation. (A) Enzyme-dependent oxygenation products of EPA converted by COX-2 (TXB3) and 5-LO (LTB5) determined in PBMC supernatants. Gray bars, PBMC were alloreactively activated; open bars, unstimulated PBMC. Means ± SEM of n = 4. (B) COX-2 expression in unstimulated monocytes. PBMC from buffy coats were incubated without or with 100 µM EPA or DHA for 24 h. Monocytes were identified by staining for CD14. Expression of COX-2 was flow-cytometrically analyzed by intracellular staining. Left: Data are expressed as means ± SEM of n = 5, a versus b: P < 0.05. Right: Representative histograms of monocytes stained for CD14 and COX-2. Marker was set in reference to the isotype control. (C) Monohydroxy products of EPA: (1) 5-HEPE, (2) 8-HEPE, (3) 9-HEPE, (4) 12-HEPE, (5) 15-HEPE, and (6) 18-HEPE. (D) Mono- (HDHA) and Trihydroxy products (Resolvin D1, RvD1) of DHA: (7) 4-HDHA, (8) 7-HDHA, (9) 8-HDHA, (10) 11-HDHA, (11) 14-HDHA, (12) 17-HDHA, and (13) 20-HDHA. Means ± SEM of n = 4.