Table 2.

Deletion of Zic3 from the heart and outflow tract does not affect viability and cardiac laterality

Nkx2.5-cre/+ X Zic3flox/flox
Stage	Total	Zic3flox/y	Zic3flox/+	Zic3flox/+; Nkx2.5-cre	Zic3flox/y; Nkx2.5-cre	P-value
9.5 dpc	71	20	16	16	19	0.87 (χ2 = 0.72)
Post-birth 3 weeks	154	42	44	34	34	0.54 (χ2 = 2.15)
Wnt1-cre/+ X Zic3flox/flox
Stage	Total	Zic3flox/y	Zic3flox/+	Zic3flox/+; Wnt1-cre	Zic3flox/y; Wnt1-cre	P-value
9.5 dpc	93	26	23	21	23	0.9 (χ2 = 0.57)
Post-birth 3 weeks	230	59	57	61	53	0.89 (χ2 = 0.62)

No observable abnormalities were found in Zic3^flox/y; Nkx2.5-cre and Zic3^flox/y; Wnt1-cre mutants. P-values were calculated from the χ² analysis.