Skip to main content
. Author manuscript; available in PMC: 2013 Mar 22.
Published in final edited form as: Chem Res Toxicol. 2008 Aug 16;21(9):1806–1813. doi: 10.1021/tx8001548

Fig. 2.

Fig. 2

Fig. 2

Fig. 2

Effect of arsenite and/or zinc on PARP-1 activity. HaCat cells were incubated with 20 μM arsenite (A) or 1 μM arsenite (B) with or without 20 μM zinc for 24 hrs. Then 0.1 mM H2O2 was incubated in the indicated groups for 5 min. The PARP-1 activity was detected by measuring the production of PAR using immunofluorescence. Scale bar = 200 μm. (C). HaCat cells were incubated with 1 μM arsenite with or without 20 μM zinc for 24 hrs. Then 0.1 mM H2O2 was incubated in the indicated groups for 5 min. The PARP-1 activities were examined functionally by HT Colorimetric PARP Assay kit. Data (n ≥ 3) were presented as means ± S.D. * p<0.05 vs indicated group in the figure.