Table 3. Dimeric structure characteristics in different crystals of GLTPsulfatide complexes.
| Mutual 22 inclination and midpoint distance† | Monomermonomer contact surface area‡ (2) | |||
|---|---|---|---|---|
| Sulfatide name§ (crystal form) | angle¶ () | t distance¶ () | Proteinprotein | Total |
| wt-GLTP | ||||
| 12:0 monoSF (1) | 76.1 | 18.2 | 1050 | 1870 |
| 12:0 monoSF (2) | 68.2 | 15.4 | 1080 | 1830 |
| 12:0 monoSF (3), chains A/B | 77.9 | 18.1 | 1090 | 1890 |
| 12:0 monoSF (3), chains D/E | 80.2 | 19.5 | 1030 | 1800 |
| 24:1 monoSF (1) (3rzn) | 77.2 | 18.2 | 1020 | 1830 |
| 24:1 GalCer (1) (2euk ††) | 75.2 | 16.7 | 1040 | 1870 |
| 12:0 diSF (1) | 75.8 | 18.4 | 960 | 1770 |
| 12:0 diSF (2) | 69.0 | 13.6 | 1120 | 2080 |
| D48V-mutant GLTP | ||||
| 12:0 monoSF (1) | 62.6 | 10.4 | 1300 | 1580 |
| 24:1 monoSF (1) (3s0i) | 64.8 | 10.8 | 1310 | 1540 |
| 12:0 diSF (1) | 65.5 | 10.7 | 1250 | 2300 |
| 12:0 diSF (4) | 63.1 | 10.8 | 1260 | 2250 |
| A47D/D48V double-mutant GLTP | ||||
| 12:0 monoSF (1) | 74.4 | 15.6 | 970 | 1810 |
| 24:1 monoSF (1) (3ric) | 75.3 | 15.7 | 1040 | 1890 |
†
The line connecting the C atoms of residues 41 and 63 was taken as the 2 helix axis.
‡
Surface-contact areas were calculated with the CCP4 suite.
§
Names as cited in the text.
¶
Interhelical angles ( angles) and midpoint distances (t distances) were calculated using the INTERHLX program (K. Yap, University of Toronto).
††
Nonsulfated ligand from Malinina et al. (2006 ▶).