Skip to main content
. Author manuscript; available in PMC: 2013 May 31.
Published in final edited form as: Brain Res. 2012 Apr 4;1457:1–12. doi: 10.1016/j.brainres.2012.03.066

Fig. 1.

Fig. 1

Reciprocal midline and paramedial expression of two major cell types in the DR provided the basis for screening for genes expressed in medio-lateral patterns. A1. Expression of tryptophan hydroxylase 2 (Tph2), a marker for 5-HT neurons, is visible at the base of the aqueduct (arrows); Tph2 cells are outlined. The outline from this image is transferred to several other sagittal sections in subsequent figures as a reference for the distribution of 5-HT cells. Coronal sections at rostral (A2.), middle (A3.), and caudal (A4.) levels: note Tph2 expressing cells are enriched on the midline (bracketed area ‘M’), with less expression paramedially. B1–4. GABAergic neurons in the DR defined by the expression of the Vesicular GABA Transporter, Slc32a1 or VGAT. In sagittal section (B1.) note the low density of expression in the circled area where Tph2-expressing cells would lie. In coronal sections at rostral (B2.), middle (B3.), and caudal (B4.) levels, VGAT expressing neurons are more abundant paramedially (bracketed area, ‘PM’) than on the midline.