Skip to main content
. 2013 Jan 17;121(12):2234–2243. doi: 10.1182/blood-2012-07-440099

Figure 4.

Figure 4

CD56+LFA-1- ILC22 cells do not become cNK cells. (A) At day 21, stage III progenitors (CD56+CD94-CD117high) were sorted on the basis of LFA-1 expression for ILC22 cells (LFA-1−) and cNK cells (LFA-1+). (B) Cells were cultured with IL-15, IL-7, SCF, and FLT3L or IL-15 alone for additional 7 days. Greater than half of the stage III cells that expressed LFA-1 at the time of sorting acquired CD94 and therefore differentiated into stage IV or V cNK cells. In contrast, stage III cells that were LFA-1− did not acquire CD94 and maintained an ILC22 phenotype in either cytokine condition. (C) LFA-1− stage III cells, but not LFA-1+ cells, produce IL-22 in response to IL-1β+23 after 7 days of culture in IL-15. In contrasts, the LFA-1+ cells express IFN-γ in response to IL-12+18. (D) After cultivation with IL-15 for 14 days, most of LFA-1+ sorted cells acquired CD94, whereas LFA-1− stage III maintained LFA-1−CD94− phenotype. Results are representative of 5 donors. D, day.