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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2012 Dec 27;57:119–131. doi: 10.1016/j.freeradbiomed.2012.12.014

Fig. 1.

Fig. 1

Antioxidant t-BHQ induces Bcl-xL gene expression. (A) Hepa-1 and HepG2 cells were treated with DMSO or antioxidant t-BHQ (50 µM) for different time periods and 60 µg cell extracts were immunoblotted with Bcl-xL and actin antibodies. The Bcl-xL band intensities were quantified and plotted (Fig. 1A, lower panels). (B) The effect of t-BHQ on Bcl-xL mRNA expression was analysed by real time quantitative PCR. (C) Hepa-1 and HepG2 cells were pre-treated with cycloheximide (30 µg/ml, 2h) and followed by treatment with DMSO or t-BHQ for indicated time periods in presence of cycloheximide. Cells were lysed and 60 µg lysates were immunoblotted with indicated antibodies. (D) Hepa-1 cells were pre-treated with 2 µg/ml of actinomycin D for 2h followed by 50 µM tBHQ+actinomycin D for indicated time periods. Bcl-xL mRNA expression was analysed by real time quantitative PCR. The data shown are mean ± S.D. of three independent experiments.